Department of Laboratory Medicine,West China Hospital of Sichuan University; Department of Clinical Laboratory,the First People′s Hospital of Neijiang;
Objective To analyze the results of meningitis/encephalitis multiple PCR(MEMP-19)in the detection of cerebrospinal fluid pathogens in patients with suspected central nervous system(CNS)infection.Methods The cerebrospinal fluid samples of 208 patients in West China Hospital of Sichuan University from October to November 2019 were selected,nucleic acid was extracted and detected by MEMP-19,and the results were analyzed,and the medical records and clinical treatment information were counted.Results Pathogens were detected in 43 out of 208 cerebrospinal fluid samples,with a total detection rate of 20.67%.The detection rate of pathogens in suspected CNS infection group was 48.53%,among which EB virus detection rate was the highest(29.41%),cryptococcus and mycobacterium tuberculosis detection rates were 17.65% and10.29%,respectively.Single infection accounted for 63.64%(21/33),double infection accounted for 30.30%(10/33),triple infection accounted for 6.06%(2/33).The pathogen detection rate of non CNS infection group was 7.14%,including 6 cases of enterovirus and 4 cases of EB virus,all of which were single infection.There was no significant difference in clinical symptoms and cerebrospinal fluid related indexes between MEMP-19 positive and negative patients in suspected CNS infection group(P>0.05).Conclusion MEMP-19 technology can quickly and accurately screen and detect a variety of pathogens in cerebrospinal fluid,which is helpful for the laboratory to provide etiological basis for central nervous system infection in time.
central nervous system infection; meningitis/encephalitis multiple PCR; cerebrospinal fluid;
 MORGENSTERN C,CABRIC S,PERKA C,et al.Synovial fluid multiplex PCR is superior to culture for detection of low-virulent pathogens causing periprosthetic joint infection[J].Diagn Microbiol Infect Dis,2018,90(2):115-119.
 EICHINGER A,HAGEN A,MEYER-BHN M,et al.Clinical benefits of introducing real-time multiplex PCR for cerebrospinal fluid as routine diagnostic at a tertiary care pediatric center[J].Infection,2019,47(1):51-58.