Analysis of immune cell infiltration pattern and survival prognosis of renal carcinoma
HUA Jinjun LI Xing WANG Jie
Department of Urology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine; Department of Anesthesiology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;
Abstract：Objective To explore the infiltration pattern of immune cells in renal carcinoma,and to analyze the correlation between infiltration pattern and survival and prognosis,as well as to predict the survival time and tumor progression.Methods Transcript data and clinical information data of renal carcinoma were downloaded from The Cancer Genome Atlas(TCGA).CIBERSORT deconvolution algorithm was used to calculate the infiltration ratio of 22 kinds of immune cells in renal carcinoma.The R package was used to draw the correlation heat map of immune cells.R package was used to analyze the pattern of immune cell infiltration and staging of renal carcinoma.The Log-Rank method was used to evaluate the relationship between the infiltration pattern of immune infiltrating cells and survival prognosis of renal carcinoma.Results The data of immunocyte infiltration in renal carcinoma was downloaded by TCGA.After CIBERSORT operation and P-value filtering,the information of immunocyte infiltration distribution in 10 normal cases and 558 cases of renal carcinoma was obtained.A total of 943 cases of renal carcinoma clinical data were downloaded from TCGA website,and 526 cases of effective renal carcinoma clinical information were obtained after screening.Compared with normal tissues,level of macrophages M2 was higher in renal carcinoma tissues(P<0.05).Levels of immature B cells,plasma cells,resting CD4+memory T cells,resting dendritic cells were lower(P<0.05).With the progression of renal carcinoma,the levels of resting NK cells,monocytes,resting mast cells and resting memory CD4+T cells decreased gradually.M0 macrophages,follicular helper T cells,CD8+T cells,activated memory CD4+T cells,plasma cells and regulatory T cells increased gradually.According to survival analysis,patients with low expression of regulatory T cells and follicular helper T cells had higher 5-year survival rates.Patients with higher level of monocytes,resting dendritic cells and mast cells resting had higher 5-year survival rates.Conclusion There are various kinds of immune cells infiltrating in the tumor microenvironment of renal carcinoma,and the immune cell infiltration mode is closely related to the survival and clinical staging of renal carcinoma.Better targeted drugs can be developed according to the immune cell infiltration mode in the future.
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